Journal: Biomolecules

Article Title: NAMPT Inhibitor and P73 Activator Represses P53 R175H Mutated HNSCC Cell Proliferation in a Synergistic Manner

doi: 10.3390/biom12030438

Figure Lengend Snippet: p73 activators can repress HNSCC growth in HNSCC cell lines. Two p73 activators, RETRA and NSC59984, were able to repress OECM-1 cell growth and, to a lesser extent, Detroit 562 cell growth. Mock was calculated as 100% to normalize for other conditions.

Article Snippet: HNSCC cells were treated with mock (DMSO only), 40 μM RETRA (a p73 activator, Santa Cruz, CA, USA), 100 μM NSC59984 (a p73 activator, Sigma-Aldrich, St. Louis, MO, USA), 10 μM EX527 (a SIRT1 inhibitor, Sigma-Aldrich, St. Louis, MO, USA), 10 μM VER-155008 (an HSP70 inhibitor, MedChemExpress, Monmouth Junction, NJ, USA) or 10 μM FK886 (an NAMPT inhibitor, MedChemExpress, Monmouth Junction, NJ, USA).

Techniques:

Journal: Biomolecules

Article Title: NAMPT Inhibitor and P73 Activator Represses P53 R175H Mutated HNSCC Cell Proliferation in a Synergistic Manner

doi: 10.3390/biom12030438

Figure Lengend Snippet: p73 activator(s) activate p73 downstream genes in OCEM-1 cells. RETRA (40 μM) or NSC59984 (100 μM) could upregulate p73 downstream genes NEU4, JAG2, LIG1 and G6PD in OECM-1 cells. Mock (DMSO only) was calculated as 1 to normalize for other conditions.

Article Snippet: HNSCC cells were treated with mock (DMSO only), 40 μM RETRA (a p73 activator, Santa Cruz, CA, USA), 100 μM NSC59984 (a p73 activator, Sigma-Aldrich, St. Louis, MO, USA), 10 μM EX527 (a SIRT1 inhibitor, Sigma-Aldrich, St. Louis, MO, USA), 10 μM VER-155008 (an HSP70 inhibitor, MedChemExpress, Monmouth Junction, NJ, USA) or 10 μM FK886 (an NAMPT inhibitor, MedChemExpress, Monmouth Junction, NJ, USA).

Techniques:

Journal: Biomolecules

Article Title: NAMPT Inhibitor and P73 Activator Represses P53 R175H Mutated HNSCC Cell Proliferation in a Synergistic Manner

doi: 10.3390/biom12030438

Figure Lengend Snippet: Synergistic repression of Detroit 562 cell growth mediated by p73 activators and NAMPT inhibitors. ( A ) RETRA, NSC59984 and NAMPT inhibitor (FK886) can inhibit Detroit 562 growth. Mock was calculated as 1 to normalize for other conditions. ( B ) The coefficient of drug interaction (CDI) was calculated as (A + B)/A×B. CDI < 1 = 1 or >1 indicates that the drugs are synergistic, additive or antagonistic, respectively. CDI < 0.7 indicates that the drug is significantly synergistic. p73 activator (RETRA) and NAMPT inhibitor (FK886) act synergistically (CDI = 0.66) to repress cell proliferation in Detroit 562 cells. Another p73 activator (NSC59984) and NAMPT inhibitor (FK886) also have synergic effects (CDI = 0.6) that reduce cell growth.

Article Snippet: HNSCC cells were treated with mock (DMSO only), 40 μM RETRA (a p73 activator, Santa Cruz, CA, USA), 100 μM NSC59984 (a p73 activator, Sigma-Aldrich, St. Louis, MO, USA), 10 μM EX527 (a SIRT1 inhibitor, Sigma-Aldrich, St. Louis, MO, USA), 10 μM VER-155008 (an HSP70 inhibitor, MedChemExpress, Monmouth Junction, NJ, USA) or 10 μM FK886 (an NAMPT inhibitor, MedChemExpress, Monmouth Junction, NJ, USA).

Techniques: